Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 3, Problem 1CA
The effectiveness of sanitation procedures in hospitals is determined by sampling surfaces for bacteria. Samples can be taken by rubbing a sterile, moistened cotton swab on a surface and inoculating a nutrient agar plate or with a Rodac environmental sampling plate. The 67-mm Rodac plates are designed so that an agar medium can be overfilled, producing a dome-shaped surface that can be pressed onto a surface for sampling microbes. As the sanitation control officer, you test each method to determine which you will use. What can you conclude from these results?
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A class of 15 students (8 males and 7 females) will need to culture an unknown bacteria for a specific activity where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to prepare.
agarplate: 25mL
Agar slant: 10mL
Broth tube: 8mL
Nutrient Agar:
Yeast extract: 2g/L
peptone: 5g/L
Sodium chloride: 5g/L
Agar powder: 15g/L
A patient is diagnosed with a urinary tract infection and a urine sample is obtained and sent to the microbiology lab. The urine sample is inoculated to agar plates and after 24 hours of incubation bacterial colonies are present on the plates.
Draw what the bacterial growth on the agar plate looks like.
Describe how you would execute this first stage from the point you are handed the nutrient broth tube containing the mixed culture, through to the appearance of two colony types on a nutrient agar plate. Assume you have all the necessary equipment and materials at your disposal. Be concise, but thorough; limit yourself to a short paragraph (1/4-1/2 page at most) – include the method and techniques; what you expect to see, and how you would avoid contamination during the process.
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Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
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