The omitted step in a Gram stain method that allow differentiation of Gram-positive and Gram-negative cells. Given: “Gram stain” “differentiation “and “ omitted step ” Introduction: In 1884, the Danish scientist Hans Christian Joachim Gram is discovered the Gram staining procedure to differentiate the bacteria into two groups, Gram-positive and Gram-negative. It is the preliminary step to identify the staining characteristics of bacteria based on the morphology and arrangement, which is observed under the light microscope. The main principle of Gram stain is used to differentiate based on the color retaining ability of the stain. Gram-positive bacteria retains crystal violet and appears violet or purple in color under the microscope. Since, the cell wall of Gram-positive bacteria contain thicker peptidoglycan layer. So, it retains the stain during ethanol decolonization step. Whereas in the Gram-negative with thinner peptidoglycan layer denatured during ethanol step and retains the secondary stain (safranin) and appears pink color under the microscopic examination.

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Alleles at the P locus control seed color. Plants which are pp have white seeds, white flowers and no pigment in vegetative parts. Plants which are P_ have black seeds, purple flowers and may have varying degrees of pigment on stems and leaves. Seed color can be assessed, visually, based on if the seed is white or not white A gene for mold resistance has been reported and we want to determine its inheritance and whether it is linked to P. For the purposes of this exercise, we will assume that resistance is controlled by a single locus M, and M_ plants are resistant and mm plants are susceptible. Resistance can be measured, under greenhouse conditions, 2 weeks after planting, by injecting each seedling with a spore suspension. After two weeks, the seedlings can be rated as resistant or susceptible, based on whether or not tissue is actively sporulating. For this exercise we will use seed and data from the F10 generation of a recombinant inbred population produced using single seed…

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Question

Chapter 3, Problem 1A

Summary Introduction

To write:

The omitted step in a Gram stain method that allow differentiation of Gram-positive and Gram-negative cells.

Given:

“Gram stain” “differentiation “and “omitted step”

Introduction:

In 1884, the Danish scientist Hans Christian Joachim Gramis discovered the Gram staining procedure to differentiate the bacteria into two groups, Gram-positive and Gram-negative. It is the preliminary step to identify the staining characteristics of bacteria based on the morphology and arrangement, which is observed under the light microscope.

The main principle of Gram stain is used to differentiate based on the color retaining ability of the stain. Gram-positive bacteria retains crystal violet and appears violet or purple in color under the microscope. Since, the cell wall of Gram-positive bacteria contain thicker peptidoglycan layer. So, it retains the stain during ethanol decolonization step. Whereas in the Gram-negative with thinner peptidoglycan layer denatured during ethanol step and retains the secondary stain (safranin) and appears pink color under the microscopic examination.

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