(a) Interpretation: The method for the separation of protein B from other proteins should be determined. Concept introduction: Isoelectric pointcan be defined as the pH at which amolecule is electrically neutral or hasno net electrical charge in the statistical mean. Molecular weight can be defined as a quantity of the sum of the atomic weight values of the atoms present in a molecule.

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Answer 1

Question

Chapter 3, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The method for the separation of protein B from other proteins should be determined.

Concept introduction:

Isoelectric pointcan be defined as the pH at which amolecule is electrically neutral or hasno net electrical charge in the statistical mean.

Molecular weight can be defined as a quantity of the sum of the atomic weight values of the atoms present in a molecule.

Interpretation Introduction

(b)

Interpretation:

The means for the review of the method of isolation of Protein B, if it carries a His-Tag at its N terminus region should be determined.

Concept introduction:

Isoelectric point can be defined as the pH at which a molecule is electrically neutral or has no net electrical charge in the statistical mean.

Molecular weight can be defined as a quantity of the sum of the atomic weight values of the atoms present in a molecule.

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carbons in each of the structures below. For instance, the central carbon of chloromethylbutane (pictured 3. A chiral carbon is a carbon that is single-bonded to four different types of groups. Identify the chiral above) is a chiral carbon. (Can you see how the groups attached to it are all chemically different?) In each of the chiral molecules below, identify all the carbons that are chiral carbons by drawing a circle around each one of them. (a) the carbohydrate glucose H O (b) the carbohydrate fructose CH₂OH 1C H-C-OH 3 HO-C-H 4 H-C-OH 5 H-C-OH 6CH₂OH D-Glucose (linear form) (c) the amino acid leucine O O H3C. HO H H- -OH CH 3 NH2 H- -OH CH₂OH OH

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3. (2 points) Your lab partner accidentally used a pen instead of a pencil to mark the baseline and label the lanes of their TLC plate. Briefly (1-2 sentences for each point) describe (a) what would happen to the ink when you develop the TLC plate; and (b) how this would affect the experiment. 1

Answer 2

Question

Chapter 3, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The method for the separation of protein B from other proteins should be determined.

Concept introduction:

Isoelectric pointcan be defined as the pH at which amolecule is electrically neutral or hasno net electrical charge in the statistical mean.

Molecular weight can be defined as a quantity of the sum of the atomic weight values of the atoms present in a molecule.

Interpretation Introduction

(b)

Interpretation:

The means for the review of the method of isolation of Protein B, if it carries a His-Tag at its N terminus region should be determined.

Concept introduction:

Isoelectric point can be defined as the pH at which a molecule is electrically neutral or has no net electrical charge in the statistical mean.

Molecular weight can be defined as a quantity of the sum of the atomic weight values of the atoms present in a molecule.

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Answer 3

Question

Chapter 3, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The method for the separation of protein B from other proteins should be determined.

Concept introduction:

Isoelectric pointcan be defined as the pH at which amolecule is electrically neutral or hasno net electrical charge in the statistical mean.

Molecular weight can be defined as a quantity of the sum of the atomic weight values of the atoms present in a molecule.

Interpretation Introduction

(b)

Interpretation:

The means for the review of the method of isolation of Protein B, if it carries a His-Tag at its N terminus region should be determined.

Concept introduction:

Isoelectric point can be defined as the pH at which a molecule is electrically neutral or has no net electrical charge in the statistical mean.

Molecular weight can be defined as a quantity of the sum of the atomic weight values of the atoms present in a molecule.

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Answer 4

Question

Chapter 3, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The method for the separation of protein B from other proteins should be determined.

Concept introduction:

Isoelectric pointcan be defined as the pH at which amolecule is electrically neutral or hasno net electrical charge in the statistical mean.

Molecular weight can be defined as a quantity of the sum of the atomic weight values of the atoms present in a molecule.

Interpretation Introduction

(b)

Interpretation:

The means for the review of the method of isolation of Protein B, if it carries a His-Tag at its N terminus region should be determined.

Concept introduction:

Isoelectric point can be defined as the pH at which a molecule is electrically neutral or has no net electrical charge in the statistical mean.

Molecular weight can be defined as a quantity of the sum of the atomic weight values of the atoms present in a molecule.

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Answer 5

Chapter 3, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The method for the separation of protein B from other proteins should be determined.

Concept introduction:

Isoelectric pointcan be defined as the pH at which amolecule is electrically neutral or hasno net electrical charge in the statistical mean.

Molecular weight can be defined as a quantity of the sum of the atomic weight values of the atoms present in a molecule.

Interpretation Introduction

(b)

Interpretation:

The means for the review of the method of isolation of Protein B, if it carries a His-Tag at its N terminus region should be determined.

Concept introduction:

Isoelectric point can be defined as the pH at which a molecule is electrically neutral or has no net electrical charge in the statistical mean.

Molecular weight can be defined as a quantity of the sum of the atomic weight values of the atoms present in a molecule.

Answer 6

Chapter 3, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The method for the separation of protein B from other proteins should be determined.

Concept introduction:

Isoelectric pointcan be defined as the pH at which amolecule is electrically neutral or hasno net electrical charge in the statistical mean.

Molecular weight can be defined as a quantity of the sum of the atomic weight values of the atoms present in a molecule.

Answer 7

Chapter 3, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The method for the separation of protein B from other proteins should be determined.

Concept introduction:

Isoelectric pointcan be defined as the pH at which amolecule is electrically neutral or hasno net electrical charge in the statistical mean.

Molecular weight can be defined as a quantity of the sum of the atomic weight values of the atoms present in a molecule.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The means for the review of the method of isolation of Protein B, if it carries a His-Tag at its N terminus region should be determined.

Concept introduction:

Isoelectric point can be defined as the pH at which a molecule is electrically neutral or has no net electrical charge in the statistical mean.

Molecular weight can be defined as a quantity of the sum of the atomic weight values of the atoms present in a molecule.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The means for the review of the method of isolation of Protein B, if it carries a His-Tag at its N terminus region should be determined.

Concept introduction:

Isoelectric point can be defined as the pH at which a molecule is electrically neutral or has no net electrical charge in the statistical mean.

Molecular weight can be defined as a quantity of the sum of the atomic weight values of the atoms present in a molecule.

Answer 10

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Answer 12

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Answer 13

Ch. 3 - Prob. 1P Ch. 3 - Prob. 2P Ch. 3 - Prob. 3P Ch. 3 - Prob. 4P Ch. 3 - Prob. 5P Ch. 3 - Prob. 6P Ch. 3 - Prob. 7P Ch. 3 - Prob. 8P Ch. 3 - Prob. 9P Ch. 3 - Prob. 10P

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