BIOCHEMISTRY 2 TERM ACCESS
BIOCHEMISTRY 2 TERM ACCESS
9th Edition
ISBN: 9781319402877
Author: BERG
Publisher: MAC HIGHER
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Chapter 3, Problem 13P
Interpretation Introduction

Interpretation:

The method in which the antibody-bound beads can be used for the purification of protein should be determined.

Concept introduction:

Affinity chromatography can be defined as a technique of isolating the biochemical mixture. The biochemical mixture is based on an extremely definite interaction between antigen and antibody, receptor and ligand, enzyme and substrate, or protein and nucleic acid.

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Please help with this Mass Spectrometry Question. Thank you For the mass spec. shown in the attached image, please determine and give the amino acid sequence of the pentapeptide. Show which end is the amino terminus and which is the carboxy terminus. How does one arrive at the solution?
Draw a tripeptide of your choosing at pH 7. Have the N-terminus on the left and the C-terminus on the right. Then: Draw a triangle around the α-carbons. Draw a box around the R-groups. Circle the atoms capable of hydrogen bonding. Highlight the atoms involved in the formation of the peptide bonds. What type of structure have you drawn? (primary, secondary, tertiary or quaternary protein structure).
2. For the flow of fluid over a flat membrane of length 10cm, determine the length-average mass transfer coefficient. The relevant properties of the system are u=0.01cm²/s, D=5 x 106 cm²/s, and v = 5.0 cm/s.
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