Nester's Microbiology: A Human Perspective
9th Edition
ISBN: 9781259709999
Author: Denise G. Anderson Lecturer, Sarah Salm, Deborah Allen
Publisher: McGraw-Hill Education
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Chapter 29, Problem 8MC
Summary Introduction
Introduction:
Landfills are a way to dispose-off waste on the land by dumping the waste under multiple layers of soil, alternating with layers of waste. This method is used to dispose-off nonhazardous waste only. Unlike the open dumping grounds and/or incineration to get rid of waste, this method offers human as well as environmental safety.
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Overview of Transformation Protocol
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Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
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-Transformation procedure:
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Add CaCl2 to both tubes.
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Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 29 Solutions
Nester's Microbiology: A Human Perspective
Ch. 29 - Describe how the BOD of a water sample is...Ch. 29 - Which step of wastewater treatment removes most of...Ch. 29 - Compare and contrast the activated sludge process...Ch. 29 -
4. What is effluent?
Ch. 29 -
5. How does a septic system work?
Ch. 29 - Prob. 6SACh. 29 - Why do water-testing procedures look for coliforms...Ch. 29 - How does the ONPG/MUG test allow a sample to be...Ch. 29 -
9. Describe two advantages of composting.
Ch. 29 - Describe the use of bioremediation in the cleanup...
Ch. 29 - Prob. 1MCCh. 29 -
2. Advanced treatment is often designed to...Ch. 29 - Which of the following is not a matching pair? a)...Ch. 29 - Prob. 4MCCh. 29 -
5. Which of the following is not a matching...Ch. 29 - Prob. 6MCCh. 29 - Prob. 7MCCh. 29 - Prob. 8MCCh. 29 - Prob. 9MCCh. 29 -
10. Synthetic compounds are most likely to be...Ch. 29 -
1. A developer is interested in building vacation...Ch. 29 -
2. A public health official is investigating...Ch. 29 -
1. Why is oil not degraded when in a natural...Ch. 29 - The accompanying figure shows the effects of...
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
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