Prescott's Microbiology
Prescott's Microbiology
11th Edition
ISBN: 9781260409062
Author: WILLEY, Joanne
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 29, Problem 3AL
Summary Introduction

To determine: The relevance of techniques using oxygen penetration and spatial distribution to determine metabolic strategies of microbial mats.

Introduction. The bacterial identification is important to ensure that the unknown bacterial strain is correctly identified and categorized to allow an easier and efficient method to detect the presence of any disease that can be caused by the organism.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
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