(a) Interpretation: The reason for the separation of DNA in the agarose gel electrophoresis and to describe the reason for the difference in the DNA in each band needs to be explained. Concept introduction: Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

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Answer 1

Question

Chapter 29, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The reason for the separation of DNA in the agarose gel electrophoresis and to describe the reason for the difference in the DNA in each band needs to be explained.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

Interpretation Introduction

(b)

Interpretation:

The types of DNA represented by the various bands.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

Interpretation Introduction

(c)

Interpretation:

The reason for the cause of slower moving DNA in the given gel electrophoresis needs to be stated.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

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In 1956, E. P. Kennedy and S. B. Weiss published their study of membrane lipid phosphatidylcholine (lecithin) synthesis in rat liver. Their hypothesis was that phosphocholine joined with some cellular component to yield lecithin. In an earlier experiment, incubating 32 P-labeled phosphocholine at physiological temperature (37 °C) with broken cells from rat liver yielded labeled lecithin. This became their assay for the enzymes involved in lecithin synthesis. Determine the optimal pH for this enzyme and characterize the enzyme activity at different pH values. -O-P-O-CH2-CH₁₂-N(CH3)3 Phosphocholine H₂C-O-C-R HC-O-C-R2 + + + Cell fraction + ? HC-O-P-O-CH₁₂-CH₂-N(CH), O Phosphatidylcholine The researchers then centrifuged the broken cell preparation to separate the membranes from the soluble proteins. They tested three preparations: whole extract, membranes, and soluble proteins. Table 1 summarizes the results. Table 1: Cell fraction requirement for incorporation of 32p-phosphocholine into…

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Answer 2

Question

Chapter 29, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The reason for the separation of DNA in the agarose gel electrophoresis and to describe the reason for the difference in the DNA in each band needs to be explained.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

Interpretation Introduction

(b)

Interpretation:

The types of DNA represented by the various bands.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

Interpretation Introduction

(c)

Interpretation:

The reason for the cause of slower moving DNA in the given gel electrophoresis needs to be stated.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

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Answer 3

Question

Chapter 29, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The reason for the separation of DNA in the agarose gel electrophoresis and to describe the reason for the difference in the DNA in each band needs to be explained.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

Interpretation Introduction

(b)

Interpretation:

The types of DNA represented by the various bands.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

Interpretation Introduction

(c)

Interpretation:

The reason for the cause of slower moving DNA in the given gel electrophoresis needs to be stated.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

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Answer 4

Question

Chapter 29, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The reason for the separation of DNA in the agarose gel electrophoresis and to describe the reason for the difference in the DNA in each band needs to be explained.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

Interpretation Introduction

(b)

Interpretation:

The types of DNA represented by the various bands.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

Interpretation Introduction

(c)

Interpretation:

The reason for the cause of slower moving DNA in the given gel electrophoresis needs to be stated.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

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Answer 5

Chapter 29, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The reason for the separation of DNA in the agarose gel electrophoresis and to describe the reason for the difference in the DNA in each band needs to be explained.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

Interpretation Introduction

(b)

Interpretation:

The types of DNA represented by the various bands.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

Interpretation Introduction

(c)

Interpretation:

The reason for the cause of slower moving DNA in the given gel electrophoresis needs to be stated.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

Answer 6

Chapter 29, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The reason for the separation of DNA in the agarose gel electrophoresis and to describe the reason for the difference in the DNA in each band needs to be explained.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

Answer 7

Chapter 29, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The reason for the separation of DNA in the agarose gel electrophoresis and to describe the reason for the difference in the DNA in each band needs to be explained.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The types of DNA represented by the various bands.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The types of DNA represented by the various bands.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

Answer 10

Interpretation Introduction

(c)

Interpretation:

The reason for the cause of slower moving DNA in the given gel electrophoresis needs to be stated.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

Answer 11

Interpretation Introduction

(c)

Interpretation:

The reason for the cause of slower moving DNA in the given gel electrophoresis needs to be stated.

Concept introduction:

Gel electrophoresis is a method where particles are differentiated according to their size and charge. Agarose is a substance used in electrophoresis in order to make a gel where the biological macromolecule will run. This process is known as agarose gel electrophoresis.

Answer 12

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Answer 13

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Answer 14

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Answer 15

Ch. 29 - Prob. 1P Ch. 29 - Prob. 2P Ch. 29 - Prob. 3P Ch. 29 - Prob. 4P Ch. 29 - Prob. 5P Ch. 29 - Prob. 6P Ch. 29 - Prob. 7P Ch. 29 - Prob. 8P Ch. 29 - Prob. 9P Ch. 29 - Prob. 10P

Solution Summary: The author describes gel electrophoresis as a method where particles are differentiated according to their size and charge.

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