Prescott's Microbiology
Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 28.2, Problem 1MI

What organisms benefit from nitrification?

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You intend to insert patched dominant negative DNA into the left half of the neural tube of a chick.  1) Which side of the neural tube would you put the positive electrode to ensure that the DNA ends up on the left side?  2) What would be the internal (within the embryo) control for this experiment?  3) How can you be sure that the electroporation method itself is not impacting the embryo?  4) What would you do to ensure that the electroporation is working? How can you tell?
Describe a method to document the diffusion path and gradient of Sonic Hedgehog through the chicken embryo. If modifying the protein, what is one thing you have to consider in regards to maintaining the protein’s function?
The following table is from Kumar et. al. Highly Selective Dopamine D3 Receptor (DR) Antagonists and Partial Agonists Based on Eticlopride and the D3R Crystal Structure: New Leads for Opioid Dependence Treatment. J. Med Chem 2016.
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