BIO 1408/09 PKG W/LS CODE
5th Edition
ISBN: 9781260370829
Author: Mader
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 28.2, Problem 1LO
Summary Introduction
To write:
Summary of the functions of the skeletal and muscular systems.
Introduction:
The muscular system refers to the system of organs comprising of smooth, skeletal, and cardiac muscles. In combination with the skeletal system, it forms the musculoskeletal system, which is accountable for the motion of the human body. In the skeletal system, the skeleton refers to the part of the body that makes the auxiliary composition of an organism. There are various kinds of the skeleton, that is, the exoskeleton, endoskeleton, cytoskeleton, and the hydroskeleton. Both the skeletal and muscular systems perform various essential functions in the human body.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 28 Solutions
BIO 1408/09 PKG W/LS CODE
Ch. 28.1 - Prob. 1LOCh. 28.1 - Prob. 2LOCh. 28.1 - Differentiate between rotational and gravitational...Ch. 28.1 - Prob. 4LOCh. 28.1 - Prob. 5LOCh. 28.1 - Summarize the stimuli that are detected by...Ch. 28.1 - Prob. 2CYPCh. 28.1 - Prob. 3CYPCh. 28.1 - Prob. 1ACh. 28.1 - Prob. 2A
Ch. 28.1 - Prob. 3ACh. 28.1 - Prob. 4ACh. 28.1 - Prob. 5ACh. 28.1 - Prob. 6ACh. 28.1 - Prob. 7ACh. 28.1 - Prob. 8ACh. 28.1 - Prob. 9ACh. 28.2 - Prob. 1LOCh. 28.2 - Prob. 2LOCh. 28.2 - 3. Describe the structure of a long bone.
Ch. 28.2 - Prob. 4LOCh. 28.2 - Prob. 5LOCh. 28.2 - Prob. 1CYPCh. 28.2 - Prob. 2CYPCh. 28.2 - Prob. 3CYPCh. 28.2 - Prob. 4CYPCh. 28.2 - Prob. 10ACh. 28.2 - Prob. 11ACh. 28.2 - Prob. 12ACh. 28.2 - Prob. 13ACh. 28.2 - Prob. 14ACh. 28.2 - Prob. 15ACh. 28 - Prob. 1BYBCh. 28 - Prob. 2BYBCh. 28 - Prob. 3BYBCh. 28 - Prob. F5.4BYBCh. 28 - Prob. F6.4BYBCh. 28 - Prob. S27.1BYBCh. 28 - 1. The two leading causes of blindness are...Ch. 28 - Prob. 2TC
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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The Skeletal System; Author: Professor Dave Explains;https://www.youtube.com/watch?v=f-FF7Qigd3U;License: Standard YouTube License, CC-BY