Lehninger Principles of Biochemistry (Instructor's)
7th Edition
ISBN: 9781319117689
Author: nelson
Publisher: MAC HIGHER
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Chapter 28, Problem 2P
Summary Introduction
To determine: The condition under which transformed cell produce β-galactosidase.
Introduction:
Operon can be defined as a set of adjacent structural genes with adjacent regulatory signals that affect transcription of structural genes. Each operon consists of structural genes, promoter, regulator, and operator.
An operator is a DNA segment that turns the gene on and off. Its interaction with proteins increases or decreases the rate of transcription from occurring and thus, makes an operon inducible or repressible. Promoter is a segment of DNA where RNA polymerase binds to begin the transcription process. Lac operon is an inducible operon in which structural genes Z, Y, and A are induced in the presence of lactose
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For CARS, which statement is not true regarding its advantages?
a) Contrast signal based on vibrational characteristics, no need for fluorescent tagging.
b) Stronger signals than spontaneous Raman.
c) Suffers from fluorescence interference, because CARS signal is at high frequency.
d) Faster, more efficient imaging for real-time analysis.
e) Higher resolution than spontaneous Raman microscopy.
Draw the major product of the Claisen condensation reaction between two molecules of this ester. Ignore
inorganic byproducts.
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1. NaOCH3/CH3OH
2. Acidic workup
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O
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The total number of carbons in the parent chain is incorrect. Review the reaction conditions including starting materials and/or
intermediate structures and recount the number of carbon atoms in the parent chain of your structure.
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- Using a cell of known pathlength b = 1.25115 x 10-3 cm, a water absorption spectrum was measured. The band at 1645 cm-1, assigned to the O-H bending, showed an absorbance, A, of 1.40. a) Assuming that water density is 1.00 g/mL, calculate the water molar concentration c (hint: M= mole/L) b) Calculate the molar absorptivity, a, of the 1645 cm-1 band c) The transmitted light, I, can be written as I= Ioexp(-xb), where x is the absorption coefficient (sometimes designated as alpha), Io is the input light, and b is the cell pathlength. Prove that x= (ln10)*x*c d) Calculate x for the 1645 cm-1 bandarrow_forwardConvert 1.38 eV into wavelength (nm) and wavenumber (cm-1) (c = 2.998 x 108 m/s; h = 6.626 x 10-34 J*s).arrow_forwardCan you help me understand the CBC method on metal bridging by looking at this problem?arrow_forward
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