Pearson eText for Laboratory Experiments in Microbiology -- Instant Access (Pearson+)
12th Edition
ISBN: 9780137546503
Author: Ted Johnson, Christine Case
Publisher: PEARSON+
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Chapter 28, Problem 1Q
Summary Introduction
To determine:
The reason why the complete and minimal nutrient replica plated.
Introduction:
Replica plating is a technique, in which secondary plates having a minimal media along with other ingredients such as antibiotics are inoculated by transferring the colonies from primary plate.
Expert Solution & Answer
Explanation of Solution
The anabolic mutants are not able to use thechemicals present in the glucose minimal salt media to grow. In case, this media lacks microbes that grew on the complete media, then it can be concluded the microbes that are not able to grow are the mutants or the auxotrophs. So, the colonies that appear in only the complete nutrient plate can be isolated for the isolation of mutants.
So, for the isolation purpose both media are important.
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Chapter 28 Solutions
Pearson eText for Laboratory Experiments in Microbiology -- Instant Access (Pearson+)
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