Campbell Biology (10th Edition)
10th Edition
ISBN: 9780321775658
Author: Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson
Publisher: PEARSON
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Chapter 27.2, Problem 2CC
Distinguish between the three mechanisms by which bac- teria can transfer DNA from one bacterial cell to another.
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For each of the following ( A & B ) provide the method of transfer and a brief explanation as to why the method would not take place under the conditions described . 1. Which method of DNA transfer between bacteria would not take place if the donor and recipient were separated by a filter with a pore size of 0.45 um or another physical barrier 2. Which method of transfer would be blocked by the presence of high concentrations of DNAase ( enzymes capable of degrading DNA ) ?
Differentiate horizontal and vertical gene transfer. Compare the mechanisms of genetic recombination in bacteria (define/contrast: transformation, conjugation, and transduction). Characterize the 4 different cell types involved in conjugation.
Describe the three different methods of horizontal gene transfer among bacteria and mention their significance. Be specific when discussing the donor versus recipient cell, and if the donor and recipient cells are still alive after each horizontal gene transfer event is complete.
Please be detailed.
Chapter 27 Solutions
Campbell Biology (10th Edition)
Ch. 27.1 - Prob. 1CCCh. 27.1 - Contrast the cellular and DNA structures of...Ch. 27.1 - Prob. 3CCCh. 27.2 - Although rare on a per gene basis, new mutations...Ch. 27.2 - Distinguish between the three mechanisms by which...Ch. 27.2 - In a rapidly changing environment, which bacterial...Ch. 27.2 - WHAT IF? If a nonpathogenic bacterium were to...Ch. 27.3 - Distinguish between the four major modes of...Ch. 27.3 - A bacterium requires only the amino acid...Ch. 27.3 - WHAT IF? Describe what you might eat for a...
Ch. 27.4 - Explain how molecular systematics and metagenomics...Ch. 27.4 - WHAT IF What would the discovery of a bacterial...Ch. 27.5 - Explain how prokaryotes, though small, can be...Ch. 27.5 - MAKE CONNECTIONS Review Figure 10.6. Then...Ch. 27.6 - Identify at least two ways that prokaryotes have...Ch. 27.6 - Prob. 2CCCh. 27.6 - Prob. 3CCCh. 27 - Describe features of prokaryotes that enable them...Ch. 27 - Mutations are rare and prokaryotes reproduce...Ch. 27 - Describe the range of prokaryotic metabolic...Ch. 27 - How have molecular data informed prokaryotic...Ch. 27 - In what ways are prokaryotes key to the survivaI...Ch. 27 - Prob. 27.6CRCh. 27 - Genetic variation in bacterial populations cannot...Ch. 27 - Photoautotrophs use (A) light as an energy source...Ch. 27 - Which of the following statements is not true? (A)...Ch. 27 - Prob. 4TYUCh. 27 - Prob. 5TYUCh. 27 - Prob. 6TYUCh. 27 - Prob. 7TYUCh. 27 - SCIENTIFIC INQUIRY INTERPRET THE DATA The...Ch. 27 - WRITE ABOUT A THEME: ENERGY In a short essay...Ch. 27 - SYNTHESIZE YOUR KNOWLEDGE Explain how the small...
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- With respect to F+ and F- bacterial matings, answer the following questions: (a) How was it established that physical contact between cells was necessary? (b) How was it established that chromosome transfer was unidirectional? (c) What is the genetic basis for a bacterium’s being F+?arrow_forwardDescribe the mechanisms by which bacterial DNA becomes compacted.arrow_forwardIdentify the most mistaken (wrong) choice: a) Transcription machinery and an enhancer can bind to the chromosome at the same time. b) Organic matters may interfere with heat treatment of bacterial growth control. ( c) Nitrocellulose can be used to filter out microorganisms from a liquid solution. d) Time to kill a bacterial culture is not proportional to the number of microbes in the culturearrow_forward
- The terms conjugation, transduction, and transformation are usedto describe three different natural forms of genetic transferbetween bacterial cells. Briefly discuss the similarities and differencesamong these processes?arrow_forwardDescribe the 3 ways in which bacteria can transfer genetic material to each other via horizontal gene transfer. Be sure to explain the following: How is DNA transferred between bacteria, is there direct contact between bacteria and whether a plasmid is involved or not.arrow_forwardExplain the following statement : a) initiation of bacteriall DNA replication is an energy requiring process b) bacterial DNA polymerase can enter the termination sequence but cannot exisarrow_forward
- Assume that there are horizontal gene transfers between two completely different bacterial species. In one case it is a plasmid that is transmitted via conjugation, in the other case it is it is a part of the bacterial chromosome that is transferred via transformation. In which of the two cases is it likely that the transferred DNA will be present? left and can function in the recipient cells? Explain the biological background to your answerarrow_forwardWhat is meant by the term site-specific recombination as used in identifying the processes that lead to the integration of temperate bacteriophages into host bacterial chromosomes during lysogeny or to the formation of specialized transducing phage?arrow_forwardMark the alternative that best explains the strategies for using tails and/or fusion proteins associated with the protein of interest. * a) fusion proteins have different functions, including increasing the molecular weight of the protein of interest and thereby facilitating the process of protein purification by affinity chromatography. b) Histidine, c-Myc and GST tails have a dual function, since they can act both in reducing the formation of inclusion bodies and in facilitating the solubility of the protein of interest. c) the introduction of fusion proteins, such as Maltose-binding protein (MBP), at the C-terminus of the protein of interest has as main objective to increase the expression yield as they facilitate the protein aggregation process. d) the introduction of tails, such as c-Myc, in the C-terminus aims to facilitate the protein purification process through the affinity chromatography methodology.arrow_forward
- Explain how bacterial conjugation has been extensively used in determining the complementing gene and bacterial gene sequence.arrow_forwardWhen an E. coli donor cell duplicates a strand of plasmid DNA, and passes this DNA strand to a recipient E. coli cell, without the use of naked DNA in solution or of a viral vector, this is: an example of horizontal gene transfer by means of lysogenic bacteriophages an example of horizontal gene transfer by means of lytic bacteriophages an example of horizontal gene transfer by means of transformation an example of horizontal gene transfer by means of transduction an example of horizontal gene transfer by means of conjugationarrow_forwardWith respect to F+ and F- bacterial matings, (a) How was it established that physical contact was necessary? (b) How was it established that chromosome transfer was unidirectional? (c) What is the genetic basis of a bacterium being F+?arrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license