
Nester's Microbiology: A Human Perspective
8th Edition
ISBN: 9780073522593
Author: Denise G. Anderson Lecturer, Sarah Salm, Deborah Allen
Publisher: McGraw-Hill Education
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Chapter 27, Problem 8SA
Summary Introduction
To review:
The use of dark-field microscopy in viewing Treponema pallidum.
Introduction:
Dark-field microscopy is a technique of microscopy in which the unscattered beam of the light from the image is excluded and thus, the background around the specimen appears dark. It is used for viewing the live and unstained specimens. Dark-field microscopy allows the visualization of live wet mounts of Treponemes obtained from the lesions of syphilis.
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8. Transcription and Translation Practice: (Video 10-1 and 10-2)
A. Below is the sense strand of a DNA gene. Using the sense strand, create the antisense
DNA strand and label the 5' and 3' ends.
B. Use the antisense strand that you create in part A as a template to create the mRNA
transcript of the gene and label the 5' and 3' ends.
C. Translate the mRNA you produced in part B into the polypeptide sequence making sure
to follow all the rules of translation.
5'-AGCATGACTAATAGTTGTTGAGCTGTC-3' (sense strand)
4
What is the structure and function of Eukaryotic cells, including their organelles? How are Eukaryotic cells different than Prokaryotic cells, in terms of evolution which form of the cell might have came first? How do Eukaryotic cells become malignant (cancerous)?
What are the roles of DNA and proteins inside of the cell? What are the building blocks or molecular components of the DNA and proteins? How are proteins produced within the cell? What connection is there between DNA, proteins, and the cell cycle? What is the relationship between DNA, proteins, and Cancer?
Chapter 27 Solutions
Nester's Microbiology: A Human Perspective
Ch. 27 - How do lactobacilli help protect the vagina from...Ch. 27 - List four factors that increase the likelihood of...Ch. 27 - What are the typical signs and symptoms of...Ch. 27 - What danger might possibly be found in a spot on...Ch. 27 - What is a clue cell?Ch. 27 - List three diseases caused by different antigenic...Ch. 27 - Prob. 7SACh. 27 - Prob. 8SACh. 27 -
9. Give two ways in which the chancre of...Ch. 27 - Prob. 10SA
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