Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 27, Problem 39P
Interpretation Introduction

(a)

Interpretation:

The amount of ATP expended by a Tour de France cyclist is to be calculated.

Concept introduction:

ATP is a nucleotide that consists of adenine, ribose, and a triphosphate unit.

ATP is the high-energy molecule that provides energy to drive many processes in living cells.

The expression to calculate the moles of a substance is as follows:

Moles of substance=Mass of substanceMolar mass of substance

Interpretation Introduction

(b)

Interpretation:

The cost to power a cyclist through the Tour de France is to be calculated.

Concept introduction:

ATP is a nucleotide that consists of adenine, ribose, and a triphosphate unit.

ATP is the high-energy molecule that provides energy to drive many processes in living cells.

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In 1956, E. P. Kennedy and S. B. Weiss published their study of membrane lipid phosphatidylcholine (lecithin) synthesis in rat liver. Their hypothesis was that phosphocholine joined with some cellular component to yield lecithin. In an earlier experiment, incubating 32 P-labeled phosphocholine at physiological temperature (37 °C) with broken cells from rat liver yielded labeled lecithin. This became their assay for the enzymes involved in lecithin synthesis. Determine the optimal pH for this enzyme and characterize the enzyme activity at different pH values. -O-P-O-CH2-CH₁₂-N(CH3)3 Phosphocholine H₂C-O-C-R HC-O-C-R2 + + + Cell fraction + ? HC-O-P-O-CH₁₂-CH₂-N(CH), O Phosphatidylcholine The researchers then centrifuged the broken cell preparation to separate the membranes from the soluble proteins. They tested three preparations: whole extract, membranes, and soluble proteins. Table 1 summarizes the results. Table 1: Cell fraction requirement for incorporation of 32p-phosphocholine into…
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