BIOLOGY
BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 27, Problem 2S
Summary Introduction

To determine:

The reason how the polysaccharide capsule found in the virulent strain of the bacteria is responsible for the cause of pneumonia in mice and justification that DNA is the genetic material as explained in the Frederick Griffith experiments.

Introduction:

Frederick Griffith conducted the experiments in mice and used two strains of bacteria for infecting the mice namely the rough and smooth strains. The rough strain of the bacteria “Streptococcus” did not cause infection in the mice whereas the smooth strain of the mice has a polysaccharide capsule layer which causes infection in the mice.

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All questions a) What is the hypothesis of Griffith’s experiment? b) Why did mouse No. 3 survived? c) Why did mouse No. 4 died? d) If Griffith had injected a fifth mouse with a combination of heat-killed R strain bacteria and heat-killed S strain bacteria, would the mouse have died? Give your reason.
Bacillus thuringiensis makes toxins that kill insects. These toxins must be applied several times during the growth season to prevent insect damage. As an alternative to repeated applications, one strategy is to apply bacteria directly to leaves.However,B. thuringiensis does not survive very long in thefield. Other bacteria, such as Pseudomonas syringae, do survive.Propose a way to alter P. syringae so it could be used as an insecticide.Discuss advantages and disadvantages of this approach compared with the repeated applications of the toxins from B. thuringiensis.
In your laboratory, you have an F − strain of E. coli that is resistant to streptomycin and is unable to metabolize lactose, but it can metabolize glucose. Therefore, this strain can grow on media that contain glucose and streptomycin, but it cannot grow on media containing only lactose. A researcher has sent you two E. coli strains in two separate tubes. One strain, let’s call it strain A, has an F factor that carries the genes that are required for lactose metabolism. On its chromosome, it also has the genes that are required for glucose metabolism. However, it is sensitive to streptomycin. This strain can grow on media containing lactose or glucose, but it cannot grow if streptomycin is added to the media. The second strain, let’s call it strain B, is an F − strain. On its chromosome, it has thegenes that are required for lactose and glucose metabolism. StrainB is also sensitive to streptomycin. Unfortunately, when strains A and B were sent to you, the labels had fallen off the…
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