Pearson eText Fundamentals of General, Organic, and Biological Chemistry -- Instant Access (Pearson+)
8th Edition
ISBN: 9780135213759
Author: John McMurry, David Ballantine
Publisher: PEARSON+
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Chapter 27, Problem 27.28AP
What is a SNP?
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Example 1:
1. Suppose an enzyme (MW = 5,000 g/mole) has a concentration of 0.05 mg/L. If the
kcat is 1 x 10 s, what is the theoretical maximum reaction velocity for the
enzyme?
A) 1050 µM/s.
B) 100 µM/s.
C) 150 μM/s.
D) 105 μM/s.
In 1956, E. P. Kennedy and S. B. Weiss published their study of membrane lipid phosphatidylcholine (lecithin) synthesis in rat
liver. Their hypothesis was that phosphocholine joined with some cellular component to yield lecithin. In an earlier experiment,
incubating 32 P-labeled phosphocholine at physiological temperature (37 °C) with broken cells from rat liver yielded labeled
lecithin. This became their assay for the enzymes involved in lecithin synthesis.
Determine the optimal pH for this enzyme and characterize the enzyme activity at different pH values.
-O-P-O-CH2-CH₁₂-N(CH3)3
Phosphocholine
H₂C-O-C-R
HC-O-C-R2
+
+
+
Cell fraction + ?
HC-O-P-O-CH₁₂-CH₂-N(CH),
O
Phosphatidylcholine
The researchers then centrifuged the broken cell preparation
to separate the membranes from the soluble proteins. They
tested three preparations: whole extract, membranes, and
soluble proteins. Table 1 summarizes the results.
Table 1: Cell fraction requirement for incorporation of
32p-phosphocholine into…
Researchers isolated an unknown substance, X, from rabbit muscle. They determined its structure from the following
observations and experiments.
(a) Qualitative analysis showed that X was composed entirely of C, H, and O. A weighed sample of X was completely oxidized
and the H2O and CO2 produced were measured. This quantitative analysis revealed that X contained 40.00% C, 6.71% H, and
53.29% O by weight.
(b) The molecular mass of X, as determined by mass spectrometry, was 90.00 atomic mass units (u).
(c) Infrared spectroscopy showed that X contained one double bond.
(d) X dissolved readily in water, and the solution demonstrated optical activity when tested in a polarimeter.
(e) The aqueous solution of X is acidic.
What is the empirical formula of X?
Chapter 27 Solutions
Pearson eText Fundamentals of General, Organic, and Biological Chemistry -- Instant Access (Pearson+)
Ch. 27.1 - Decode the following sequence of letters to find...Ch. 27.3 - Prob. 27.1CIAPCh. 27.3 - Prob. 27.2CIAPCh. 27.3 - Prob. 27.3CIAPCh. 27.3 - Prob. 27.2KCPCh. 27.4 - Prob. 27.3PCh. 27.4 - A restriction enzyme known as EcoRI cuts DNA in...Ch. 27.4 - Prob. 27.5PCh. 27.5 - Classify the following activities according to the...Ch. 27.5 - Prob. 27.4CIAP
Ch. 27.5 - Prob. 27.5CIAPCh. 27.5 - Prob. 27.6CIAPCh. 27 - What steps are necessary in the mapping of the...Ch. 27 - Prob. 27.8UKCCh. 27 - List the four types of noncoding DNA (see Section...Ch. 27 - In general, what are the differences between...Ch. 27 - What is recombinant DNA? How can it be used to...Ch. 27 - Identify some major potential benefits of the...Ch. 27 - Prob. 27.13APCh. 27 - Prob. 27.14APCh. 27 - Prob. 27.15APCh. 27 - Prob. 27.16APCh. 27 - Prob. 27.17APCh. 27 - Prob. 27.18APCh. 27 - Prob. 27.19APCh. 27 - You may have heard of Dolly, the cloned sheep...Ch. 27 - Prob. 27.21APCh. 27 - Prob. 27.22APCh. 27 - What is the role of the enzyme telomerase? In what...Ch. 27 - Prob. 27.24APCh. 27 - Prob. 27.25APCh. 27 - Prob. 27.26APCh. 27 - Prob. 27.27APCh. 27 - What is a SNP?Ch. 27 - How are SNPs linked to traits in individual human...Ch. 27 - List some potential biological effects of SNPs.Ch. 27 - Prob. 27.31APCh. 27 - Prob. 27.32APCh. 27 - Prob. 27.33APCh. 27 - Prob. 27.34APCh. 27 - Prob. 27.35APCh. 27 - Prob. 27.36APCh. 27 - Prob. 27.37APCh. 27 - Prob. 27.38APCh. 27 - In the formation of recombinant DNA. a restriction...Ch. 27 - Give the sequence of unpaired bases that would be...Ch. 27 - Are the following base sequences sticky or not...Ch. 27 - Prob. 27.42APCh. 27 - Prob. 27.43APCh. 27 - Provide two examples of genetically engineered...Ch. 27 - Prob. 27.45APCh. 27 - Why is the field of bioethics so important in...Ch. 27 - Prob. 27.47CPCh. 27 - Prob. 27.48CPCh. 27 - Prob. 27.49CPCh. 27 - Prob. 27.50CPCh. 27 - What is a restriction endonuclease?Ch. 27 - Prob. 27.52CPCh. 27 - Prob. 27.53GPCh. 27 - One of the most actively pursued areas in genomics...Ch. 27 - Prob. 27.55GP
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