Biology
Biology
12th Edition
ISBN: 9780078024269
Author: Sylvia Mader, Michael Windelspecht
Publisher: McGraw-Hill Education
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Chapter 27, Problem 14A
Summary Introduction

Introduction:

Cell suspension culture is a technique that allows the extraction of the chemical from plant cells. These chemicals are secondary metabolites that can be genetically modified. These cells can cause the production of chemicals similar to those that are naturally produced by plants.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
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