PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 2818440045677
Author: WILLEY
Publisher: MCG
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Chapter 26.3, Problem 1MI
Why is the ϕX174 genome considered plus stranded?
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In a clinical context, a scientist is working with a viral DNA which is about 24000bps
long. There are two known variants of the virus that share almost the same DNA but
for a final fragment; with reference to Figure Q2b, the regions A and B are conserved
in both variants, while the region C differs and is either 320bps (variant 1) or 380bps
(variant 2). The scientist wants to set up a procedure to identify the variant they are
dealing with.
Viral dsDNA
(i)
(ii)
(iii)
Stable region (A)
Variable region (C)
Figure Q2b
Known sequence (B)
5-GACCTCAATGTCCAGCGGTACGCTCATAAA-3'
3'-CTGGAGTTACAGGTCGCCATGCGAGTATTT-5'
The scientists want to design a primer to amplify the variable region and to do
so, they sequence a small fragment (sequence B) the conserved region close
to the variable region C. Why is the scientist targeting a region outside of the
fragment of interest?
[3]
The sequence of the fragment B is reported in Figure Q2b. Suggest a primer
that can efficiently target this region and…
In the following gel showing stained bands of the Alu insertion sequence, what is the genotype
of individual 2?
941 bp
641 bp->>>
1 2 3 4 5 6
Homozygous for the 641 bp sequence that does not contain in the Alu insertion
Heterozygous, containing one 941 bp sequence and one 641 bp sequence
O Homozygous for the 941 bp sequence containing the Alu insertion
this is the worst figure i have ever seen. Evidently A and B are different but the same color? From what I understand A would be a sliding clamp, and B is Primase? Is F rna primer (I think personally) or representing the SSBP? this is the worst diagram ever.
the words that are for labeling are as follows: DNA polymerase III, sliding clamp, helicase, single-stranded binding protein (SSBPs), topoisomerase, RNA primer, newly synthesized DNA, primase, and DNA ligase.
Chapter 26 Solutions
PRESCOTT'S MICROBIOLOGY
Ch. 26.1 - List some characteristics used in classifying...Ch. 26.1 - Prob. 2CCCh. 26.2 - Prob. 1MICh. 26.2 - Why do you think T4 evolved to initiate DNA...Ch. 26.2 - What function does HMC glycosylation serve?Ch. 26.2 - Explain why the T4 genome is circularly permuted.Ch. 26.2 - Prob. 1.2CCCh. 26.2 - How is a prophage induced to become active again?Ch. 26.2 - Describe the roles of cII, CIII, repressor (CI),...Ch. 26.2 - How do the temperate phages Mu and P1 differ from...
Ch. 26.2 - How is the envelope of this virus formed? How does...Ch. 26.2 - Why do cold sores recur throughout the lifetime of...Ch. 26.2 - In what part of the host cell does a herpesvirus...Ch. 26.2 - Many small DNA viruses rely on host enzymes for...Ch. 26.3 - Why is the X174 genome considered plus stranded?Ch. 26.3 - Why is it necessary for some ssDNA viruses to...Ch. 26.3 - Prob. 2CCCh. 26.3 - How do parvoviruses trick the host DNA polymerase...Ch. 26.4 - The rotavirus genome encodes 12 proteins. Suggest...Ch. 26.4 - Describe the life cycle of 6 phage. What makes...Ch. 26.4 - Prob. 3CCCh. 26.4 - In what ways are the life cycles of 6 and...Ch. 26.5 - Where in the host does the plus-strand RNA genome...Ch. 26.5 - How do some plus-strand viruses use polyproteins...Ch. 26.5 - What is an IRES? Why is it important?Ch. 26.5 - Prob. 3CCCh. 26.6 - How does that use of a segmented genome by...Ch. 26.6 - Prob. 2CCCh. 26.7 - Prob. 1MICh. 26.7 - Prob. 1CCCh. 26.7 - Prob. 2CCCh. 26.7 - Prob. 3CCCh. 26.8 - Prob. 1CCCh. 26.8 - Trace the HBV multiplication cycle, paying...Ch. 26 - Prob. 1RCCh. 26 - Prob. 2RCCh. 26 - Prob. 3RCCh. 26 - Prob. 4RCCh. 26 - No temperate RNA phages have yet been discovered....Ch. 26 - The choice between lysogeny and lysis is...Ch. 26 - Prob. 3ALCh. 26 - You are studying RNA viruses and have discovered a...Ch. 26 - Prob. 5ALCh. 26 - Prob. 6AL
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