Inquiry into Life
Inquiry into Life
14th Edition
ISBN: 9780073525525
Author: Mader, Sylvia S./
Publisher: McGraw-Hill College
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Chapter 26, Problem 5A
Summary Introduction

Introduction:

Polymerase chain reaction (PCR) is a common laboratory technique that used to prepare many copies (millions or billions!) of the desired gene or a particular region of DNA. This desire gene can be the interest of experiment. Typically, the aim of PCR is to produce enough of desired DNA region for analysis or another experiment purpose such as gel electrophoresis used to visualized PCR amplified DNA and use for cloning into a plasmid for further experiments. Thus, PCR has been used in many areas due to its advance and amplification feature in biology and medicine, including molecular biology research, medical diagnostics, and even some branches of ecology.

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The shape of radishes may be long (SL/SL), oval (SL/SS), or round (SS/SS), and the color of radishes may be red (CR/CR), purple (CR/CW) or white (CW/CW).  If a long, red radish plant is crossed with a round, white plant, what will be the appearance of the F1 and F2 generations?
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Question #3: In the KeyGene paper, the authors state that it would be useful if pollen from an apomict would transmit apomixis-inducing genes to the female in the cross (assuming the pollen is viable). Assuming there was just one gene conferring gametophytic obligate apomixis, and that the two parents are inbreds, what would be the consequences of such a cross if: a) The apomixis was a dominant trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the parental cross. b) The apomixis was a recessive trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the…
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