EBK LEHNINGER PRINCIPLES OF BIOCHEMISTR
EBK LEHNINGER PRINCIPLES OF BIOCHEMISTR
7th Edition
ISBN: 8220103662253
Author: nelson
Publisher: MAC HIGHER
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Chapter 26, Problem 1P

(a)

Summary Introduction

To determine: The time duration that is required by E.coli RNA polymerase to synthesize the primary transcript for the E.coli gene that encode for lactose metabolism have 5300 bp Lac operon.

Introduction:

Lac operon is a cell regulatory mechanism that is used to operate the metabolism of lactose in E. coli. The lactose is catabolized by a set of genes known as lac operon. The set of genes present in the lac operon are regulated by regulator gene.

RNA polymerase is a subunit that will catalyze the transcription process in which RNA polymer is synthesize from the DNA template

(a)

Expert Solution
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Explanation of Solution

Explanation:

The elongation of the RNA transcript in E.coli will run at the rate of about 50 to 90 nucleotide per second.

Time needed by primary transcript is:

T=5300nucleotides5090nucleotidespersecond=59106sec

The time can be rounded to 60-100 sec.

Conclusion

Conclusion:

Thus, the time required for primary transcript is 60-100 sec.

(b)

Summary Introduction

To determine: The length of the transcriptional bubble formed by RNA polymerase.

Introduction:

RNA polymerase is a subunit that will catalyze the transcription process in which RNA polymer is synthesize from the DNA template. RNA polymerase is majorly found in all living organism that takes parts in the central dogma process.

(b)

Expert Solution
Check Mark

Explanation of Solution

Explanation:

The elongation of the RNA transcript in E.coli will run at the rate of about 50 to 90 nucleotide per second.

The distance travelled by the replication bubble in 10 sec is:

=(10sec)×(5090nuceotide/sec)=500900nucleotides

Conclusion

Conclusion:

Distance travelled by the replication bubble in 10 sec is 500-900nucleotides.

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