Restriction enzyme also recognized as a restriction endonuclease, which is produced by bacteria and used to cleave DNA at specific sites along the molecule. They recognize a short and specific sequence of nucleotide bases of DNA that known as recognition site or recognition sequences and randomly distributed throughout the DNA. Restriction enzyme helps to eliminate infecting organisms by cleaving the foreign DNA in the bacterial cell. As well as restriction enzymes are employed in the biological laboratory to manipulate fragments of DNA by insertion of new DNA fragment that contains specific genes. Thus, restriction enzymes are indispensable tools of recombinant DNA technology or genetic engineering.

Question

Want to see the full answer?

Answer 1

Question

Chapter 26, Problem 1A

Summary Introduction

Introduction:

Restriction enzyme also recognized as a restriction endonuclease, which is produced by bacteria and used to cleave DNA at specific sites along the molecule. They recognize a short and specific sequence of nucleotide bases of DNA that known as recognition site or recognition sequences and randomly distributed throughout the DNA.

Restriction enzyme helps to eliminate infecting organisms by cleaving the foreign DNA in the bacterial cell. As well as restriction enzymes are employed in the biological laboratory to manipulate fragments of DNA by insertion of new DNA fragment that contains specific genes. Thus, restriction enzymes are indispensable tools of recombinant DNA technology or genetic engineering.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

7. Aerobic respiration of a protein that breaks down into 12 molecules of malic acid. Assume there is no other carbon source and no acetyl-CoA. NADH FADH2 OP ATP SLP ATP Total ATP Show your work using dimensional analysis here: 3

For each of the following problems calculate the following: (Week 6-3 Video with 6-1 and 6-2) Consult the total catabolic pathways on the last page as a reference for the following questions. A. How much NADH and FADH2 is produced and fed into the electron transport chain (If any)? B. How much ATP is made from oxidative phosphorylation (OP), if any? Feed the NADH and FADH2 into the electron transport chain: 3ATP/NADH, 2ATP/FADH2 C. How much ATP is made by substrate level phosphorylation (SLP)? D. How much total ATP is made? Add the SLP and OP together. 1. Aerobic respiration using 0.5 mole of glucose? NADH FADH2 OP ATP SLP ATP Total ATP Show your work using dimensional analysis here:

Aerobic respiration of one lipid molecule. The lipid is composed of one glycerol molecule connected to two fatty acid tails. One fatty acid is 12 carbons long and the other fatty acid is 18 carbons long in the figure below. Use the information below to determine how much ATP will be produced from the glycerol part of the lipid. Then, in part B, determine how much ATP is produced from the 2 fatty acids of the lipid. Finally put the NADH and ATP yields together from the glycerol and fatty acids (part A and B) to determine your total number of ATP produced per lipid. Assume no other carbon source is available. 18 carbons fatty acids 12 carbons glycerol . Glycerol is broken down to glyceraldehyde 3-phosphate, a glycolysis intermediate via the following pathway shown in the figure below. Notice this process costs one ATP but generates one FADH2. Continue generating ATP with glyceraldehyde-3-phosphate using the standard pathway and aerobic respiration. glycerol glycerol-3- phosphate…