Cleavage of the given cyclopeptide by trypsin and chymotrypsin gives the same fragments or not need to be identified. Concept introduction: Enzymatic cleavage is a useful method for identifying the sequence of amino acid residue in a large peptide. This method uses enzymes for hydrolysis of the peptide bonds. These enzymes are known as peptidases. Some of the frequently used enzyme is trypsin and chymotrypsin. Trypsin selectively hydrolyzes the peptide bond at the carboxyl side of arginine and lysine. Chymotrypsin selectively hydrolyzes the peptide bond at the carboxyl end of amino acids containing aromatic side chains. The amino acids which have aromatic side chains are phenylalanine, tyrosine and tryptophan. To explain : To explain whether the fragments obtained by hydrolysis of cyclopeptide using trypsin and chymotrypsin are same or different.

Question

Want to see the full answer?

Answer 1

Question

Chapter 25.5, Problem 33ATS

Interpretation Introduction

Interpretation:

Cleavage of the given cyclopeptide by trypsin and chymotrypsin gives the same fragments or not need to be identified.

Concept introduction:

Enzymatic cleavage is a useful method for identifying the sequence of amino acid residue in a large peptide. This method uses enzymes for hydrolysis of the peptide bonds. These enzymes are known as peptidases. Some of the frequently used enzyme is trypsin and chymotrypsin.

Trypsin selectively hydrolyzes the peptide bond at the carboxyl side of arginine and lysine.

Chymotrypsin selectively hydrolyzes the peptide bond at the carboxyl end of amino acids containing aromatic side chains. The amino acids which have aromatic side chains are phenylalanine, tyrosine and tryptophan.

To explain : To explain whether the fragments obtained by hydrolysis of cyclopeptide using trypsin and chymotrypsin are same or different.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

: At a certain temperature, the equilibrium constant K for the following reaction is 1.58 × 10-12 N2(g) + O2(g) = 2 NO(g) Use this information to complete the following table. Suppose a 38. L reaction vessel is filled with 0.93 mol of N2 and 0.93 mol of O2. What can you say about the composition of the mixture in the vessel at equilibrium? There will be very little N2 and O2. There will be very little NO. What is the equilibrium constant for the following reaction? Be sure your answer has the correct number of significant digits. 2 NO(g) N2(9)+02(9) What is the equilibrium constant for the following reaction? Be sure your answer has the correct number of significant digits. 3 N2(9)+302(g) 6 NO(g) Neither of the above is true. K = ☐ K = ☐ ☐ X10 Х D ? 000 18 Ar B

when performing the reaction that involves 2 equivalents of 3-(diethylamino)-phenol and Phthalic anhydride with sulfuric acid and water react to form rhodamine b where the Phthalic anhydride cleaves in acid and how does Excessive Washing (w/ Base) & Subsequent Resonance Structure get affected

3. The strongest acid of the following compounds is ___.A. p-nitrophenol; B. m-nitrophenol; C. o-chlorophenol;D. p-methoxyphenol; E. o-methylphenol Please explain your steps and thought process. Thank you!