EBK BIOLOGY
4th Edition
ISBN: 8220102797376
Author: BROOKER
Publisher: YUZU
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Chapter 25.4, Problem 2CC
Summary Introduction
To determine: The relationship between the total number of Hox genes in an animal species and its morphological complexity
Introduction: The Hox genes are homeotic genes that are present in all animals and play a significant role in development. These genes are expressed along the anteroposterior axis during the development of the embryo.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
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Add CaCl2 to both tubes.
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Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 25 Solutions
EBK BIOLOGY
Ch. 25.1 - Can you think of another example of two different...Ch. 25.1 - Prob. 1BCCh. 25.1 - Is temporal isolation an example of a prezygotic...Ch. 25.1 - Prob. 3CCCh. 25.2 - Prob. 1BCCh. 25.2 - Prob. 1EQCh. 25.2 - Prob. 2EQCh. 25.2 - Prob. 3EQCh. 25.2 - Prob. 1CCCh. 25.2 - Prob. 2CC
Ch. 25.4 - Evo-Devo: Evolutionary Developmental Biology...Ch. 25.4 - Prob. 2CCCh. 25.4 - Prob. 1BCCh. 25.4 - Prob. 3CCCh. 25 - Macroevolution refers to evolutionary changes that...Ch. 25 - Prob. 2TYCh. 25 - Prob. 3TYCh. 25 - Prob. 4TYCh. 25 - Prob. 5TYCh. 25 - Prob. 6TYCh. 25 - Prob. 7TYCh. 25 - Prob. 8TYCh. 25 - Prob. 9TYCh. 25 - Prob. 10TYCh. 25 - What is the key difference between prezygotic and...Ch. 25 - What are the key differences between gradualism...Ch. 25 - Prob. 3CQCh. 25 - Prob. 1COQCh. 25 - Discuss the type of speciation (allopatric or...
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- Which of the following sequencing techniques can identify down to the strain level? O Multilocus sequence typing Genomic fingerprinting Whole genome sequencing OSNP analysis All of the abovearrow_forwardWhat is the "gold standard" that is currently applied to species designations in microbiology? 97% between species: 50% among whole genome 90% between species: 75% among whole genome 99% between species; 97% among whole genome 97% between species: 70% among whole genome Onone of the abovearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have decided to send your sample off for sequencing. You need to determine which type of sequencing to use for the preliminary identification of your species. You decide that, for now, you only need to be able to identify the family and genus levels. Which type of sequencing do you think is the most appropriate? O Genomic Fingerprinting O Whole Genome Sequencing O 16S rDNA Sequencing O DNA-DNA hybridization Nextarrow_forward
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