In aldopentose, the carbon which determines the D and L configurations has to be determined. Concept Introduction: The D and L configurations can be defined by the –OH group present on the penultimate carbon. If the –OH group is present on the right side of the carbon atom, it is said to be D-form (dextrorotatory form). If the –OH group is present on the left side of the carbon atom, it is said to be L-form (levorotatory form).

Question

(a 4 shows scanning electron microscope (SEM) images of extruded actions of packing bed for two capillary columns of different diameters, al 750 (bottom image) and b) 30-μm-i.d. Both columns are packed with the same stationary phase, spherical particles with 1-um diameter. A) When the columns were prepared, the figure shows that the column with the larger diameter has more packing irregularities. Explain this observation. B) Predict what affect this should have on band broadening and discuss your prediction using the van Deemter terms. C) Does this figure support your explanations in application question 33? Explain why or why not and make any changes in your answers in light of this figure. Figure 4 SEM images of sections of packed columns for a) 750 and b) 30-um-i.d. capillary columns.³

Answer 1

Question

Chapter 25, Problem 25.9P

Interpretation Introduction

Interpretation:

In aldopentose, the carbon which determines the D and L configurations has to be determined.

Concept Introduction:

The D and L configurations can be defined by the –OH group present on the penultimate carbon. If the –OH group is present on the right side of the carbon atom, it is said to be D-form (dextrorotatory form). If the –OH group is present on the left side of the carbon atom, it is said to be L-form (levorotatory form).

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