MICROBIOLOGY: EVOLV.SCI.-W/ACCESS>CI<
4th Edition
ISBN: 9780393622805
Author: SLONCZEWSKI
Publisher: Norton custom
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Chapter 25, Problem 16RQ
Summary Introduction
To review:
The mechanism used by bacteria to determine the environment around them.
Introduction:
The pathogen that enters the host invades the part in which it multiplies. It will stop the immune system of the host to act against it, using various mechanisms. They use many pathways to deliver toxins into the cell. The pathogens are also able to sense the environment around them using a special system.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 25 Solutions
MICROBIOLOGY: EVOLV.SCI.-W/ACCESS>CI<
Ch. 25.1 - Prob. 1TQCh. 25.4 - Prob. 1TQCh. 25.4 - Prob. 2TQCh. 25.4 - Prob. 3TQCh. 25.4 - Prob. 4TQCh. 25.5 - Prob. 1TQCh. 25.6 - Prob. 1TQCh. 25.6 - Prob. 2TQCh. 25.6 - Prob. 3TQCh. 25.6 - Prob. 4TQ
Ch. 25.7 - Prob. 1TQCh. 25 - Prob. 1RQCh. 25 - Prob. 2RQCh. 25 - Prob. 3RQCh. 25 - Prob. 4RQCh. 25 - Prob. 5RQCh. 25 - Prob. 6RQCh. 25 - Prob. 7RQCh. 25 - Prob. 8RQCh. 25 - Prob. 9RQCh. 25 - Prob. 10RQCh. 25 - Prob. 11RQCh. 25 - Prob. 12RQCh. 25 - Prob. 13RQCh. 25 - Prob. 14RQCh. 25 - Prob. 15RQCh. 25 - Prob. 16RQCh. 25 - Prob. 17RQCh. 25 - Prob. 1TQCh. 25 - Prob. 2TQCh. 25 - Prob. 3TQCh. 25 - Prob. 4TQCh. 25 - Prob. 5TQ
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