Chapter 25, Problem 13P

Interpretation Introduction

Interpretation:

The altered sequence of amino acid which is present in the affected part of double mutation involving one amino acid to be added (shown below)

Wild Type - $\mathrm{...}\text{Lys}-\text{Ser}-\text{Pro}-\text{Ser}-\text{Leu}-\text{Asn}-\text{Ala}\mathrm{...}$

Double Mutant - $\mathrm{...}\text{Lys}-\text{Ser}-\text{Val}-\text{His}-\text{His}-\text{Leu}-\text{Met}-\text{Ala}\mathrm{...}$

The consistency of the above result with the codon assignment found in vitro should be discussed.

Concept introduction:

The genetic stability is increased by the genetic code. Each genetic codon consists of sequences of three nucleotides and each codon codes to one amino acid. Around 20 functional amino acids are present in the cell.

Genetic code is also liable to minimize the point mutations. The point mutations could affect the functions of protein. Any mutation in the genetic code could lead to a change in the sequence of amino acid in the protein. This sometimes results in serious health complications like sickle cell anemia.