Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
7th Edition
ISBN: 9781464126116
Author: David L. Nelson, Michael M. Cox
Publisher: W. H. Freeman
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Chapter 25, Problem 12P
Summary Introduction

To determine: How the colonies of Salmonella typhimurium auxotrophs grew in histidine deficient medium.

Introduction:

Histidine is an essential amino-acid metabolite required for the growth of the strain of Salmonella typhimurium. It is added in the nutrient growth medium during the start of nutrient culture.

Summary Introduction

To determine: The growth of Salmonella typhimurium colonies after addition of 0.4 µg 2-aminoanthracene.

Introduction:

Chemical 2-aminoanthracene contains an aromatic ring which is used for testing reverse mutation in strains of bacteria.

Summary Introduction

To determine: The carcinogenicity of chemical 2-aminoanthracene.

Introduction:

The 2-aminoanthracene is an aromatic cyclic ring chemical which can cause point or frameshift mutations in the genome of the species of bacteria.

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Background Freezing isn't the only challenge in cryopreservation - thawing can be just as difficult. A microwave oven seems like a nice solution, since it deposits energy quickly and microwaves are non-ionizing radiation (they do not cause DNA mutation). However, water absorbs microwaves more effectively than ice does, meaning that the portion of an organ that has already melted will get warmer at a higher rate than the remaining ice – the opposite of what we want! - The transmission of radiation through a weakly absorbing material such as ice or water can be modeled by Beer's law, which assumes that the rate of absorption at a depth x is proportional to the local radiation intensity I(x) times an absorption coefficient, which is often written as μ or a or just µ). Noting that absorption decreases the intensity, we can write a differential equation a Solving the differential equation with the boundary condition on the surface being gives the relationship For a standard microwave oven…
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