BIOLOGY
5th Edition
ISBN: 9781265202859
Author: BROOKER
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Chapter 25, Problem 10TY
Summary Introduction
Introduction: The horizontal gene transfer or movement of the genetic sequences from the parent to the offspring takes place separately and not along with the transmission of DNA. It is helpful in causing variations in the progeny and results in the evolution of the organism.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 25 Solutions
BIOLOGY
Ch. 25.1 - Prob. 1CSCh. 25.1 - Prob. 1CCCh. 25.2 - Can two different species have more than one...Ch. 25.2 - Prob. 2CCCh. 25.3 - What shared derived character is common to the...Ch. 25.3 - Prob. 2CCCh. 25.3 - Prob. 3CCCh. 25.3 - Prob. 1EQCh. 25.3 - Prob. 2EQCh. 25.3 - Prob. 3EQ
Ch. 25.4 - Prob. 1CSCh. 25.5 - Prob. 1CCCh. 25 - Prob. 1TYCh. 25 - Which of the following is the correct order of the...Ch. 25 - Prob. 3TYCh. 25 - Which of the following characteristics is (are)...Ch. 25 - Which of the following occur at branch points, or...Ch. 25 - Prob. 6TYCh. 25 - Prob. 7TYCh. 25 - A goal of modern taxonomy is to a. classify all...Ch. 25 - The concept that the preferred hypothesis is the...Ch. 25 - Prob. 10TYCh. 25 - Prob. 1CQCh. 25 - What is a molecular clock? How is it useful in the...Ch. 25 - Core Concept: Evolution What are some advantages...Ch. 25 - Prob. 1COQCh. 25 - Prob. 2COQ
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forward
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