CUSTOM PRESCOTT'S MICROBIOLOGY
CUSTOM PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 9781266032844
Author: WILLEY
Publisher: MCG
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Chapter 24.4, Problem 4CC
Summary Introduction

Any eukaryotic organism which is not a plant, animal, or fungus is called a protist. It includes the protozoans, certain oomycetes, algae, and slime molds. In 1866, Ernst Haeckel first used the term prostista. Depending on the similarities to the “higher” kingdom, it was traditionally subdivided into many groups include protozoa and protophyta. Protozoa is a unicellular organism and it can be subdivided depending on features such as motility includes Ciliophora, Flagellate, Sporozoa, and amoeba.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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Microbial Nutrition and Growth; Author: Scientist Cindy;https://www.youtube.com/watch?v=rK3UkyWjkl8;License: Standard YouTube License, CC-BY