Connect With Learnsmart Labs Online Access For Prescott's Microbiology
11th Edition
ISBN: 9781260408997
Author: Joanne Willey
Publisher: Mcgraw-hill Higher Education (us)
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Chapter 24.4, Problem 2MI
What are the functions of the micronucleus and macronucleus?
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Chapter 24 Solutions
Connect With Learnsmart Labs Online Access For Prescott's Microbiology
Ch. 24.1 - Prob. 1CCCh. 24.1 - Prob. 2CCCh. 24.1 - Prob. 3CCCh. 24.1 - Prob. 4CCCh. 24.1 - Prob. 5CCCh. 24.1 - Describe vesicular, ovular, and chromosomal...Ch. 24.1 - Why has taxonomic classification of protists been...Ch. 24.2 - Prob. 1MICh. 24.2 - What are the functions of the stigma and the...Ch. 24.2 - Prob. 1CC
Ch. 24.2 - What is the function of the plasmalemma and...Ch. 24.2 - Prob. 3CCCh. 24.3 - Prob. 1MICh. 24.3 - Prob. 2MICh. 24.3 - Prob. 1CCCh. 24.3 - Prob. 2CCCh. 24.3 - Prob. 3CCCh. 24.4 - Prob. 1MICh. 24.4 - What are the functions of the micronucleus and...Ch. 24.4 - How does the trigger for sexual reproduction...Ch. 24.4 - Prob. 1CCCh. 24.4 - Compare how radiolaria use axopodia with the way...Ch. 24.4 - Prob. 3CCCh. 24.4 - Prob. 4CCCh. 24.4 - Describe conjugation as it occurs in ciliates....Ch. 24.4 - Prob. 6CCCh. 24.4 - Prob. 7CCCh. 24.4 - Prob. 8CCCh. 24.4 - Prob. 9CCCh. 24.5 - Prob. 1MICh. 24.5 - Compare the morphology of members of the genus...Ch. 24.5 - Prob. 2CCCh. 24 - Prob. 1RCCh. 24 - Prob. 2RCCh. 24 - Prob. 3RCCh. 24 - Prob. 4RCCh. 24 - Prob. 5RCCh. 24 - Prob. 6RCCh. 24 - Protist encystment is usually triggered by changes...Ch. 24 - Vaccine development for diseases caused by...Ch. 24 - Prob. 3ALCh. 24 - Benthic foraminifera inhabit marine sediments. It...Ch. 24 - Prob. 5AL
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
- In oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donorarrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have shipped your samples off for sequencing and are now waiting for the results. Out of curiosity (and maybe boredom...) you decide to test your culture for the Catalase and Oxidase enzymes. Upon testing your sample for catalase, you don't see any bubbles; however, you do see a color change to purple during the Oxidase test. What results can you conclude from this? O Catalase-/ Oxidase + O Catalase +/ Oxidase + Catalase + / Oxidase- O Catalase / Oxidase - O None of the abovearrow_forwardWhich of the following is not a strength of using 16S rRNA for phylogenetic analyses? OA. It's cheap OB. It's easy to do C. It can be used to identify all the way down to the strain level OD. Both A & B OE. None of the abovearrow_forward
- Why are molecular approaches important to the field of microbial taxonomy and phylogeny? Phylogenetic inferences based on molecular approaches provide the most robust analysis of microbial evolution currently available. It allows for the collection of a large and accurate dataset from many organisms Almost no fossil record was left by microbes when compared to plants and animals All of the above None of the abovearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forward
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