BIOLOGY (LOOSELEAF)-W/CONNECT
BIOLOGY (LOOSELEAF)-W/CONNECT
12th Edition
ISBN: 9781260692181
Author: Raven
Publisher: MCG
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Chapter 24.3, Problem 2LO
Summary Introduction

To determine: The reason that horizontal gene transfer can complicate evolutionary hypotheses.

Introduction: The gene transfer between the bacteria takes place through the process of transformation. The naked DNA molecule is transferred from one bacterium that is resistant to ampicillin to the bacterium that is sensitive to ampicillin. As there is no other aid like a conjugation tuber or a phage particle, the genetic material transfers directly and brings about the transformation of the bacteria

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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