Microbiology: A Systems Approach
5th Edition
ISBN: 9781259706615
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Chapter 24.3, Problem 12AYP
Summary Introduction
To determine:
The basic process used to perform metagenomic analysis of the environment.
Concept introduction:
Metagenomics refers to the direct analysis of genomes contained with an environmental sample. Metagenomics provides the powerful tool for the environmentally friendly design of nanoparticles. It also provides access to the functional gene composition of microbial communities and thus provides a much description to a phylogenetic analysis.
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19. On the diagram below
a. Label the three pictures as: DNA; polypeptide; or RNA.
b. Label the arrows as: translation or transcription/RNA processing.
c. Add the following details to the diagram.
Promoter region
TATA box
Transcription start site
Transcription terminator
Intron (A,B,C,D)
Exons (1,2,3,4,5)
Splice sites
5' cap
5' UTR (untranslated region)
3' poly A tail
3' UTR (untranslated region)
Translational start (AUG)
Translational stop (UGA, UAG, or UAA)
N and C ends of polypeptide
0000
Match the letter labels in the figure below to the terms. Some letter
labels are not used.
MNNNNNNIN
M
C
B
A
M
D
F
E
H
K
G
8
The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
Chapter 24 Solutions
Microbiology: A Systems Approach
Ch. 24.1 - Define microbial ecology.Ch. 24.1 - Summarize why our view of the abundance of...Ch. 24.1 - Prob. 3AYPCh. 24.1 - Prob. 4AYPCh. 24.1 - Prob. 5AYPCh. 24.2 - List five important elements of biogeochemical...Ch. 24.2 - Diagram a carbon cycle.Ch. 24.2 - Prob. 8AYPCh. 24.2 - Prob. 9AYPCh. 24.2 - Describe the process of nitrogen fixation, and...
Ch. 24.2 - Prob. 11AYPCh. 24.3 - Prob. 12AYPCh. 24.3 - Prob. 13AYPCh. 24.3 - Prob. 14AYPCh. 24.3 - Prob. 15AYPCh. 24.3 - Prob. 16AYPCh. 24.3 - Prob. 17AYPCh. 24.4 - Prob. 18AYPCh. 24.4 - Prob. 19AYPCh. 24 - Which of the following is not a major subdivision...Ch. 24 - Prob. 2MCQCh. 24 - Prob. 3MCQCh. 24 - Prob. 4MCQCh. 24 - Root nodules contain ______, which can ________....Ch. 24 - Prob. 6MCQCh. 24 - Prob. 7MCQCh. 24 - Prob. 8MCQCh. 24 - Prob. 9MCQCh. 24 - Recent studies reveal that a. 100% of...Ch. 24 - Prob. 11TFCh. 24 - Prob. 12TFCh. 24 - Prob. 13TFCh. 24 - A plastisphere is a specialized ball used when...Ch. 24 - Prob. 15TFCh. 24 - Prob. 1CTQCh. 24 - a. Outline the general characteristics of a...Ch. 24 - Summarize the role microbes play in the cycling of...Ch. 24 - Many people use animal manure to fertilize their...Ch. 24 - PCBs are human-made pollutants that are not...Ch. 24 - Prob. 1VCCh. 24 - From chapter 8, figure 8.24. What process does...Ch. 24 - Prob. 1CM
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