Microbiology: An Introduction
11th Edition
ISBN: 9780321733603
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: Benjamin Cummings
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Chapter 24, Problem 8R
Summary Introduction
To review:
To write the procedures and positive results of the tuberculin test and indication of positive test.
Introduction:
Tuberculosis is caused by Mycobacterium tuberculosis. It is gram positive bacteria which is coinfected with HIV. Tuberculin skin test is done for the confirmation of tuberculosis in a patient who has the symptoms of TB. Reddish patch appear on the skin when a positive results comes to a patient.
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Chapter 24 Solutions
Microbiology: An Introduction
Ch. 24 - DRAW IT Show the locations of the following...Ch. 24 - Compare and contrast mycoplasmal pneumonia and...Ch. 24 - Prob. 3RCh. 24 - Complete the following table.Ch. 24 - Under what conditions can the saprophytes...Ch. 24 - Prob. 6RCh. 24 - List the causative agent, mode of transmission,...Ch. 24 - Prob. 8RCh. 24 - Prob. 9RCh. 24 - Prob. 10R
Ch. 24 - Prob. 1ACh. 24 - Prob. 2ACh. 24 - Prob. 3ACh. 24 - Prob. 1MCQCh. 24 - No bacterial pathogen can be isolated from the...Ch. 24 - Prob. 3MCQCh. 24 - Match the following choices to the culture...Ch. 24 - Prob. 5MCQCh. 24 - Match the following choices to the culture...Ch. 24 - Prob. 7MCQCh. 24 - Prob. 8MCQCh. 24 - Prob. 9MCQCh. 24 - Prob. 10MCQCh. 24 - In August, a 24-year-old man from Virginia...Ch. 24 - Prob. 2CAECh. 24 - Prob. 3CAE
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- State the principle of the antiglobulin test. Differentiate monoclonal from polyclonal and monospecific from polyspecific antihuman globulin (AHG) reagents.arrow_forwardWhat other infections or conditions can be detected by an ELISA?arrow_forwardDraw the steps of Qualitative Slide Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR: SCREEN AND TITER 1. Bring all reagents and test samples to room temperature before use. 2. Make a 1:20 dilution of the patient serum by pipetting 0.1 ml of patient serum into 1.9 mL of 1X diluent or saline in a test tube. Pipet 50 µl of the diluted sample onto one test circle on the slide. 3. Place one drop (about 50 mL) of positive control onto a separate test circle and one drop of negative control onto another test circle. 4. Invert the latex reagent bottle several times to mix the beads into the suspension. Add one drop of well-shaken reagent to each test area of the slide. 5. Mix each test area with a disposable stirrer, spreading each mixture over the entire section. Use a clean disposable stirrer for each test area. 6. Rock the slide gently with a rotary motion for 2 minutes and observe immediately for agglutination.arrow_forward
- Provide reasons for false-positive nontreponemal test results.arrow_forwardDepending on the purpose, the typhoid fever can be diagnosed in the laboratory by either of the following method, i) Widal, ii. Blood culture and iii. Nucleic acid based identification method. Write a brief essay comparing these three diagnostic tests. Your answer must contain the basis of identification of each test, what does it mean by positive and negative results and which test do you think to be useful for the identification of active patient and which test is/are useful for the screening of previously infected case. Which test is prerequisite to perform the antibiotic susceptibility pattern?arrow_forwardDraw these steps of Qualitative Slide Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR: SCREEN AND TITER 1. Bring all reagents and test samples to room temperature before use. 2. Make a 1:20 dilution of the patient serum by pipetting 0.1 ml of patient serum into 1.9 ml of 1X diluent or saline in a test tube. Pipet 50 µul of the diluted sample onto one test circle on the slide. 3. Place one drop (about 50 mL) of positive control onto a separate test circle and one drop of negative control onto another test circle. 4. Invert the latex reagent bottle several times to mix the beads into the suspension. Add one drop of well-shaken reagent to each test area of the slide. 5. Mix each test area with a disposable stirrer, spreading each mixture over the entire section. Use a clean disposable stirrer for each test area. 6. Rock the slide gently with a rotary motion for 2 minutes and observe immediately for agglutination.arrow_forward
- What is the indication of violet blue color in biuret test? Explain in 1-3 sentencesarrow_forwardWhat are the factors to consider when handling a stool sample? Also, in 6-7 sentences, discuss the techniques used in microscopic examination of stool.arrow_forwardDescribe the method, time frame, and rationale for the tuberculinskin test. What component of the immune response does thistest detect?arrow_forward
- Describe and explain the serum protein profile of patients with suspected myeloma, as seen using non-denaturing polyacrylamide gel electrophoresis.arrow_forwarda. Seropositivity means having a blood serum that tests positive. Explain why it may or may not develop at the same rate in all patients exposed to the same microbe. b. Would a high rate of false-positives decrease the sensitivity or specificity of a serological test?arrow_forwardSerological testing:a. Describe the key principles behind serological testing in the identification of an unknown organism.arrow_forward
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