EBK PRINCIPLES OF BIOLOGY
EBK PRINCIPLES OF BIOLOGY
2nd Edition
ISBN: 8220103675338
Author: BROOKER
Publisher: YUZU
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Chapter 24, Problem 5TY
Summary Introduction

Introduction:

Microbiome consists of all the genetic material of the entire collection of microbes in a specific environment. Species of archaea, bacteria, protists and fungi are considered to be microorganisms. Some microbiomes are found within physical systems. Some microbiomes are associated with living organisms. These living organisms are known as hosts. The host is an organism which allows another organism to live inside or near their body and be in a symbiotic relationship. Humans are microbiome hosts.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
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