PRIN. OF LIFE (LL)-W/ACHIEVE
PRIN. OF LIFE (LL)-W/ACHIEVE
3rd Edition
ISBN: 9781319492069
Author: HILLIS
Publisher: MAC HIGHER
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Chapter 23.2, Problem 4RA

a.

Summary Introduction

To describe: The reason due to which only root apical meristem cells contain radioactive thymidine at 0 hours of incubation.

Introduction: Tips of roots and shoots consist of apical meristem tissues. They can differentiate into different types of cells. Division and growth in meristematic tissue lead to the growth of plants.

b.

Summary Introduction

To calculate: The percentage of radioactive cells, plotting radioactive cells percentage over time.

Introduction: Apical meristems are present at tips of root and shoots divide to form new cells and increase the length of roots and shoots.

c.

Summary Introduction

To analyze: Rise and fall in the percentage in radioactive cells in each region.

Introduction: Apical meristem divides continuously that result in the growth of the plant. Meristematic tissues are present at tips of roots and shoots.

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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
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