Essentials of Biology
Essentials of Biology
4th Edition
ISBN: 9780078024221
Author: Sylvia S. Mader Dr., Michael Windelspecht
Publisher: McGraw-Hill Education
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Chapter 23.1, Problem 2A
Summary Introduction

Introduction:

The circulatory system refers to the cardiovascular system of the body. This system includes heart and the blood vessels. Blood vessels are basically of three types; arteries, capillaries, and veins. The heart is a muscular organ of the circulatory system that pumps blood throughout the body and the blood vessels carry the blood within them. Circulatory system provides oxygen and nutrients to all the cells of the body through blood vessels.

The circulatory system consists of a pulmonary circuit and a systemic circuit. Pulmonary circuit lies between lungs and heart, while the systemic circuit lies between the heart and the body.

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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
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The Cardiovascular System: An Overview; Author: Strong Medicine;https://www.youtube.com/watch?v=Wu18mpI_62s;License: Standard youtube license