Microbiology: An Introduction
Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Chapter 23, Problem 3CAE
Summary Introduction

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There are five patients undergone thoracotomy surgery for aortic value replacement, out of five patients three were developed bacteremia through Enterobacter cloacae due to unsterilized manometer which was culture positive for Enterobacter cloacae.

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What are the common pathogens isolated from stool samples? What is the difference between a coliform bacterium and a noncoliform enteric bacterium? What diagnostic test differentiates Proteus and Providencia species from other Enterobacteriaceae? How would you differentiate between serotypes of E. coli? Are the gram-negative enteric bacilli fastidious organisms? Would they survive well outside of the body? If so, what significance would this have in their transmission? Why is serotyping particularly important in Salmonella infections and typhoid fever?
EXPLAIN THE PURPOSE OF THE METHYL RED AND VOGES-PROSKAUER TESTS IN DETERMINING THE IDENTITY OF GRAM NEGATIVE ORGANISMS IN THE FAMILY ENTEROBACTERIACEAE. INCLUDE IN YOUR ANSWER WHY METHYL RED IS USED RATHER THAN PHENOL RED. WHAT ORGANISM IN THE FAMILY IS USED AS A CONTROL WHEN RUNNING THE METHYL TEST. IF THE METHYL RED TEST IS NEGATIVE, EXPLAIN WHY THE VOGES-PROSKAUER TEST IS RUN AND INCLUDE WHAT COLOR CHANGE INDICATES A POSITIVE TEST. ACCOUNT FOR THE COLOR DIFFERENCES IN THE NEGATIVE VOGES-PROSKAURER TESTS.  WHAT REAGENTS (COMMON NAME) WERE USED IN THE VOGES -PROSKAUER TEST.
Three to five days after eating Thanksgiving dinner at a restaurant, 112 people developed fever and gastroenteritis. The dinner consisted of Roast Turkey, turkey giblet soup, mashed potatoes and crushed giblet gravy. The gravy was not refrigerated and was served the entire Thanksgiving day. The analysis of leftover food showed the same bacteria as was isolated from patients. a) What was the source of illness? b) What was the most likely microbe causing this illness? c) Was this an infection or an intoxication?
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