Chapter 23, Problem 23.42P

(a)

Interpretation Introduction

Interpretation:

$\text{2}\text{.5}\text{mg}$ of unknown mixture were injected in twice the injection volume but you might not achieve the same resolution, reason for this observation has to be given.

Concept introduction:

Purnell equation for resolution:

The relationship between $\text{H}\text{and}\text{α}$ is given by Purnell equation,

$\begin{array}{l}\text{Resolution}\text{=}\frac{\sqrt{\text{N}}}{\text{4}}\frac{\left(\text{α}\text{-}\text{1}\right)}{\text{α}}\left(\frac{{\text{k}}_{\text{2}}}{\text{1}\text{+}\text{}{\text{k}}_{\text{2}}}\right)\\ \text{Where,}\\ \text{N}\text{-}\text{number}\text{of}\text{theoretical}\text{plates}\\ \text{α}\text{-}\text{relative}\text{retention}\text{of}\text{the}\text{two}\text{peaks}\\ {\text{k}}_{\text{2}}\text{-}\text{retention}\text{factor}\text{of}\text{the}\text{more}\text{retained}\text{component}\end{array}$

(b)

Interpretation Introduction

Interpretation:

$\text{5}\text{mg}$ of unknown mixture were injected in the original injection volume but you might not achieve the same resolution, reason for this observation has to be given.

Concept introduction:

Overloading:

Which is responsible for the gradual rise and sudden fall of the peak.

Types of overloading:

• Mass overload (loading too much analyte)
• Volume overload (loading too much liquid)

When the sample size is decreased, the overloading is decreases and the peak becomes more symmetric.