HUMAN BIOLOGY + VIRTUAL PAC
HUMAN BIOLOGY + VIRTUAL PAC
16th Edition
ISBN: 9781264359028
Author: Mader
Publisher: MCG/CREATE
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Chapter 22.3, Problem 1CYP
Summary Introduction

To determine:

The polymerase chain reaction and DNA cloning to make gene copies.

Introduction:

The cloning or copy of the gene is required to determine the functions of the gene. The characteristic involves size, tissue distribution and expression of the gene. The cloning helps in the production of a high amount of protein.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
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