BIOLOGY
BIOLOGY
5th Edition
ISBN: 9781264104680
Author: BROOKER
Publisher: MCG
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Chapter 22, Problem 9TY
Summary Introduction

Introduction: Evolution refers to the changes which can be transferred from the parents to the offsprings. Historically, anatomies of extinct and modern species were compared to understand evolution, but after the arrival of noble methods for analyzing DNA sequences, molecular processes can be taken into consideration to understand evolution. Molecular processes such as mutations and horizontal gene transfer are also some of the factors contributing to evolution.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
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