Biology Now (Core Edition)
Biology Now (Core Edition)
1st Edition
ISBN: 9780393906332
Author: Anne Houtman, Megan Scudellari, Cindy Malone, Anu Singh-Cundy
Publisher: W. W. Norton & Company
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Chapter 22, Problem 8TQ
Summary Introduction

The adrenal glands are a pair of endocrine glands located above the kidneys. The adrenal glands secrete three hormones such as cortisol, adrenaline, and noradrenaline. The release of adrenaline into the blood stream can produce various rapid physiological responses of the body, for example, boosting glucose levels. However, adrenaline suppresses the immune response in the body.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
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