Nester's Microbiology: A Human Perspective
Nester's Microbiology: A Human Perspective
8th Edition
ISBN: 9780073522593
Author: Denise G. Anderson Lecturer, Sarah Salm, Deborah Allen
Publisher: McGraw-Hill Education
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Chapter 22, Problem 1A
Summary Introduction

To review:

A child gets affected bychicken poxand is not allowed to attend the school to avoid an outbreak. However, it has been argued that an outbreak would benefit the children that would be affected by it in a long run. Determine the pros and cons of chicken pox on its outbreak.

Introduction:

Chickenpox is an infectious disease popularly called varicella. It is caused by the varicella-zoster virus (VZV). The causative agent of chickenpoxbelongs to the family of herpesvirus in appearance. Chickenpox is a common childhood infection while the adults can also be infected by VZV. When a pregnant woman catches the infection, it can be very serious for unborn babies.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
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