CUSTOM PRESCOTT'S MICROBIOLOGY
CUSTOM PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 9781266032844
Author: WILLEY
Publisher: MCG
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Chapter 21.3, Problem 2CC
Summary Introduction

Deinococci are rod-shaped, spherical, non-motile bacteria. These bacteria are frequently seen in tetrads or pairs. They are aerobic heterotrophs that produce acid only from a few sugars. These bacteria are Gram-positive and contain L-ornithine in their peptidoglycan layer but lack teichoic acid. S-layer is present in their membrane envelope.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
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