Biology
Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 21.1, Problem 1CS
Summary Introduction

To explain: The general properties of the plasmid.

Introduction: Genome is defined as the complete genetic material that is present in an organism. It consists of both the coding as well as the non-coding parts of DNA. The study of the genome is known as genomics. Genomics contains an important technique by which multiple and exact copies of a gene can be produced. This technique is known as gene cloning.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
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