
Concept explainers
(a) Interpretation:
If the original molecule is linear or circular should be explained.
Concept introduction:
A restriction enzyme or restriction endonuclease is a protein which can recognize a particular short sequence of
(b) Interpretation:
A map of restriction sites that is consistent with the data should be drawn, indicating the number of bases between restriction sites.
Concept introduction:
A restriction enzyme or restriction endonuclease is a protein which can recognize a particular short sequence of nucleotide and cuts the DNA only at that particular site, which is known as the target sequence or restriction site. Restriction enzymes are given their names after the organisms from which they are isolated. The number of cleavages in the DNA sequence made by a particular restriction enzyme can be determined by the specific number of restriction sites present for that specific enzyme in the DNA fragment. The recognition sequences are randomly distributed all through the DNA. Different species of bacteria make restriction enzymes which have the ability to recognize different nucleotide sequences.
(c) Interpretation:
If there are any other maps that would be consistent with this data should be explained.
Concept introduction:
A restriction enzyme or restriction endonuclease is a protein which can recognize a particular short sequence of nucleotide and cuts the DNA only at that particular site, which is known as the target sequence or restriction site. Restriction enzymes are given their names after the organisms from which they are isolated. The number of cleavages in the DNA sequence made by a particular restriction enzyme can be determined by the specific number of restriction sites present for that specific enzyme in the DNA fragment. The recognition sequences are randomly distributed all through the DNA. Different species of bacteria make restriction enzymes which have the ability to recognize different nucleotide sequences.
(d) Interpretation:
The process used to locate the cleavage sites unambiguously with respect to each other should be explained.
Concept introduction:
A restriction enzyme or restriction endonuclease is a protein which can recognize a particular short sequence of nucleotide and cuts the DNA only at that particular site, which is known as the target sequence or restriction site. Restriction enzymes are given their names after the organisms from which they are isolated. The number of cleavages in the DNA sequence made by a particular restriction enzyme can be determined by the specific number of restriction sites present for that specific enzyme in the DNA fragment. The recognition sequences are randomly distributed all through the DNA. Different species of bacteria make restriction enzymes which have the ability to recognize different nucleotide sequences.

Want to see the full answer?
Check out a sample textbook solution
Chapter 21 Solutions
BIOCHEMISTRY BOOKS ALC&MOD MST/ET PKG
- Draw the reaction between sphingosine and arachidonic acid. Draw out the full structures.arrow_forwardDraw both cis and trans oleic acid. Explain why cis-oleic acid has a melting point of 13.4°C and trans-oleic acid has a melting point of 44.5°C.arrow_forwardDraw the full structure of the mixed triacylglycerol formed by the reaction of glycerol and the fatty acids arachidic, lauric and trans-palmitoleic. Draw the line structure.arrow_forward
- Draw out the structure for lycopene and label each isoprene unit. "Where is lycopene found in nature and what health benefits does it provide?arrow_forwardWhat does it mean to be an essential fatty acid? What are the essential fatty acids?arrow_forwardCompare and contrast primary and secondary active transport mechanisms in terms of energy utilisation and efficiency. Provide examples of each and discuss their physiological significance in maintaining ionic balance and nutrient uptake. Rubric Understanding the key concepts (clearly and accurately explains primary and secondary active transport mechanisms, showing a deep understanding of their roles) Energy utilisation analysis ( thoroughly compares energy utilisation in primary and secondary transport with specific and relevant examples Efficiency discussion Use of examples (provides relevant and accurate examples (e.g sodium potassium pump, SGLT1) with clear links to physiological significance. Clarity and structure (presents ideas logically and cohesively with clear organisation and smooth transition between sections)arrow_forward
- 9. Which one of the compounds below is the major organic product obtained from the following reaction sequence, starting with ethyl acetoacetate? 요요. 1. NaOCH2CH3 CH3CH2OH 1. NaOH, H₂O 2. H3O+ 3. A OCH2CH3 2. ethyl acetoacetate ii A 3. H3O+ OH B C D Earrow_forward7. Only one of the following ketones cannot be made via an acetoacetic ester synthesis. Which one is it? Ph کہ A B C D Earrow_forward2. Which one is the major organic product obtained from the following reaction sequence? HO A OH 1. NaOEt, EtOH 1. LiAlH4 EtO OEt 2. H3O+ 2. H3O+ OH B OH OH C -OH HO -OH OH D E .CO₂Etarrow_forward
- what is a protein that contains a b-sheet and how does the secondary structure contributes to the overall function of the protein.arrow_forwarddraw and annotate a b-sheet and lable the hydrogen bonding. what is an example that contains the b-sheet and how the secondary structure contributes to the overall function of your example protein.arrow_forwardFour distinct classes of interactions (inter and intramolecular forces) contribute to a protein's tertiary and quaternary structures. Name the interaction then describe the amino acids that can form this type of interaction. Draw and annotate a diagram of the interaction between two amino acids.arrow_forward
- BiochemistryBiochemistryISBN:9781319114671Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.Publisher:W. H. FreemanLehninger Principles of BiochemistryBiochemistryISBN:9781464126116Author:David L. Nelson, Michael M. CoxPublisher:W. H. FreemanFundamentals of Biochemistry: Life at the Molecul...BiochemistryISBN:9781118918401Author:Donald Voet, Judith G. Voet, Charlotte W. PrattPublisher:WILEY
- BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage LearningBiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage LearningFundamentals of General, Organic, and Biological ...BiochemistryISBN:9780134015187Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. PetersonPublisher:PEARSON





