Chapter 21, Problem 44P

Interpretation Introduction

(a)

Interpretation:

The reason for a “smear” in lane 1 of the western blot gel should be explained.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only based on their molecular mass.

Western blot is visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis is transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(b)

Interpretation:

The significance of the decrease in high molecular weight bands in lane 2 should be explained.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only the basis of their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(c)

Interpretation:

Significance of the difference between lane 2 and 3 should be explained

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis is transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(d)

Interpretation:

A plausible reason for no difference between lanes 3 and 4 should be suggested.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and charges the proteins both negatively and evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(e)

Interpretation:

The 66 kDabands observed in all the lanes treated with amylase, despite the fact that the cells were treated differently should be justified.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and charges the proteins both negatively and evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.