Chapter 21, Problem 31EQ

Summary Introduction

To review:

The expected results of electrophoretic mobility shift assay (EMSA) done on the following samples:

Lane 1. 600 base pair (bp) fragment containinga glucocorticoid response element (GRE) and glucocorticoid receptor.

Lane 2. 600 bp segment having a GRE, glucocorticoid receptor, and glucocorticoid hormone.

Lane 3. 600 bp segment with a GRE and CREB (cyclic adenosine monophosphate (cAMP) response element binding protein) protein.

Lane 4. 700 bp segment having a cAMP response element (CRE) and CREB protein.

Lane 5. 700 bp fragment having a CRE along with CREB protein, and hormone which causes phosphorylation of CREB protein.

Lane 6. 700 bp segment with a CRE as well as glucocorticoid receptor, and glucocorticoid hormone.

Introduction:

Electrophoretic mobility shift assay is done to study the interaction of protein and deoxyribonucleic acid (DNA) or the interaction of protein and ribonucleic acid (RNA). This technique is used to find out whether the given protein or the mixture of proteins can bind to a specific DNA or RNA fragment.