Chapter 21, Problem 25EQ

Summary Introduction

To analyze:

The results of western blot of a gene on mutation in varying conditions like:

1. Normal gene from a plant.

2. Gene without promoter.

3. Heterozygous mutation in which one gene is normal and other is short due to introduction of a stop codon at 112 position.

4. Homozygous mutation in which stop codon is inserted at 112 base pair (bp).

5. Homozygous mutation in which phenylalanine is changed to leucine.

Introduction:

In western blotting, the fragments of polypeptides that are produced within the cell are detected by using the antibodies as probes. The proteins from cell are purified and are loaded in the electrophoresis. These proteins then are observed by autoradiography or fluorescence.