EBK EXPLORING CHEMICAL ANALYSIS
EBK EXPLORING CHEMICAL ANALYSIS
5th Edition
ISBN: 8220101443908
Author: Harris
Publisher: MAC HIGHER
Question
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Chapter 21, Problem 21.19P

(a)

Interpretation Introduction

Interpretation:

Plate height has to be calculated.

Concept Introduction:

Expression to compute plate height by van Deemter equation is as follows:

  H=A+B/u+Cu

Here,

A denotes coefficient of eddy diffusion.

B denotes coefficient of longitudinal diffusion.

C denotes coefficient of mass transfer.

u denotes linear velocity of the mobile phase mL/min.

H denotes plate height in mm.

(b)

Interpretation Introduction

Interpretation:

Number of plates has to be calculated on the column.

Concept Introduction:

Expression to compute number of plates from H is as follows:

  N=LH

Here,

N denotes number of theoretical plate.

L denotes length of chromatographic column.

H denotes plate height.

(c)

Interpretation Introduction

Interpretation:

Value of w1/2 has to be calculated.

Concept Introduction:

Expression to compute w1/2 from number of theoretical plate is as follows:

  w1/2=(5.55 tr2N)1/2

Here,

tr denotes analyte’s  retention time.

w1/2 denotes peak width at half-height.

N denotes number of theoretical plate.

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