Connect With Learnsmart Labs Online Access For Prescott's Microbiology
11th Edition
ISBN: 9781260408997
Author: Joanne Willey
Publisher: Mcgraw-hill Higher Education (us)
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Chapter 2.1, Problem 1MI
Summary Introduction
The focal length of a system is the distance between the center of the curved mirror or a lens and its focus. In microscopy, this term refers to the amount of distance which is required between the top surface of the object or sample and the objective lens, so as to view the image through the microscope that is in-focus.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
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Add CaCl2 to both tubes.
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Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 2 Solutions
Connect With Learnsmart Labs Online Access For Prescott's Microbiology
Ch. 2.1 - Prob. 1MICh. 2.1 - Prob. 1CCCh. 2.1 - Prob. 2CCCh. 2.1 - Prob. 3CCCh. 2.2 - Prob. 1MICh. 2.2 - Prob. 2MICh. 2.2 - Prob. 3MICh. 2.2 - Prob. 1CCCh. 2.2 - Prob. 2CCCh. 2.2 - Prob. 3CC
Ch. 2.2 - Prob. 4CCCh. 2.2 - Prob. 5CCCh. 2.2 - Prob. 6CCCh. 2.3 - Prob. 1MICh. 2.3 - Prob. 1CCCh. 2.3 - Retrieve, Infer, Apply 2. Why would basic dyes be...Ch. 2.3 - Prob. 3CCCh. 2.3 - Prob. 4CCCh. 2.4 - Prob. 1MICh. 2.4 - Prob. 1CCCh. 2.4 - Prob. 2CCCh. 2.4 - Prob. 3CCCh. 2.4 - Prob. 4CCCh. 2.5 - Prob. 1MICh. 2.5 - Prob. 1CCCh. 2.5 - Prob. 2CCCh. 2 - Prob. 1RCCh. 2 - Prob. 2RCCh. 2 - Prob. 3RCCh. 2 - Prob. 1ALCh. 2 - Prob. 2ALCh. 2 - Prob. 4AL
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